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mouse-anti-neun (neuronal marker) mab377  (Millipore)


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    Millipore mouse-anti-neun (neuronal marker) mab377
    Mouse Anti Neun (Neuronal Marker) Mab377, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neuronal+marker+neun/mouse+anti+neun/pmc12210276-474-13-11
    Average 90 stars, based on 1 article reviews
    mouse-anti-neun (neuronal marker) mab377 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Differential effects of immunotherapy with antibodies targeting α-synuclein oligomers and fibrils in a transgenic model of synucleinopathy
    Article Snippet: Immunohistochemistry, neuropathological and image analysis Analysis of α-syn accumulation was performed using vibratome-cut free-floating coronal brain sections (40 μm). .. Brain sections were incubated overnight at 4 °C with a polyclonal antibody against total α-syn (1:500, affinity-purified rabbit polyclonal, Millipore) ( Games et al., 2013 ; Masliah et al., 2000 ) in the presence or absence of proteinase K (PK; 10 μg/ml for 15 min) or non-immune IgG controls (background levels), a neuronal marker (NeuN, mouse monoclonal, Millipore 1:2500), an astroglial cell marker (glial fibrillary acidic protein [GFAP], mouse monoclonal, Millipore 1:2500), a microglial cell marker (Iba1; goat polyclonal, Abcam, 1:5000). followed by incubations with secondary antibodies biotinylated (1:100, Vector Laboratories, Inc., Burlin-game, CA), Avidin D-HRP (1:200, ABC Elite, Vector) and detection with 3,3′-diaminobenzidine ( Masliah et al., 2011 ). ..

    Affinity Purification:

    Article Title: Differential effects of immunotherapy with antibodies targeting α-synuclein oligomers and fibrils in a transgenic model of synucleinopathy
    Article Snippet: Immunohistochemistry, neuropathological and image analysis Analysis of α-syn accumulation was performed using vibratome-cut free-floating coronal brain sections (40 μm). .. Brain sections were incubated overnight at 4 °C with a polyclonal antibody against total α-syn (1:500, affinity-purified rabbit polyclonal, Millipore) ( Games et al., 2013 ; Masliah et al., 2000 ) in the presence or absence of proteinase K (PK; 10 μg/ml for 15 min) or non-immune IgG controls (background levels), a neuronal marker (NeuN, mouse monoclonal, Millipore 1:2500), an astroglial cell marker (glial fibrillary acidic protein [GFAP], mouse monoclonal, Millipore 1:2500), a microglial cell marker (Iba1; goat polyclonal, Abcam, 1:5000). followed by incubations with secondary antibodies biotinylated (1:100, Vector Laboratories, Inc., Burlin-game, CA), Avidin D-HRP (1:200, ABC Elite, Vector) and detection with 3,3′-diaminobenzidine ( Masliah et al., 2011 ). ..

    Marker:

    Article Title: Differential effects of immunotherapy with antibodies targeting α-synuclein oligomers and fibrils in a transgenic model of synucleinopathy
    Article Snippet: Immunohistochemistry, neuropathological and image analysis Analysis of α-syn accumulation was performed using vibratome-cut free-floating coronal brain sections (40 μm). .. Brain sections were incubated overnight at 4 °C with a polyclonal antibody against total α-syn (1:500, affinity-purified rabbit polyclonal, Millipore) ( Games et al., 2013 ; Masliah et al., 2000 ) in the presence or absence of proteinase K (PK; 10 μg/ml for 15 min) or non-immune IgG controls (background levels), a neuronal marker (NeuN, mouse monoclonal, Millipore 1:2500), an astroglial cell marker (glial fibrillary acidic protein [GFAP], mouse monoclonal, Millipore 1:2500), a microglial cell marker (Iba1; goat polyclonal, Abcam, 1:5000). followed by incubations with secondary antibodies biotinylated (1:100, Vector Laboratories, Inc., Burlin-game, CA), Avidin D-HRP (1:200, ABC Elite, Vector) and detection with 3,3′-diaminobenzidine ( Masliah et al., 2011 ). ..

    Article Title: Microarray analysis of expression of cell death-associated genes in rat spinal cord cells exposed to cyclic tensile stresses in vitro
    Article Snippet: .. Previously we have documented [ ] that ~71% of cells isolated from the spinal cord using this methodology are positive for the neuronal marker NeuN (Chemicon International, Temecula, CA). ..

    Article Title: Prefrontal Cortical Dysfunction After Overexpression of Histone Deacetylase 1
    Article Snippet: .. ... To determine which of the major cell populations express the transgene (which was driven by a generic cytomegalovirus promoter) when packaged into the AAV9 capsid, we employed co-localization studies on brain sections of mice injected with the AAV9-LacZ reporter by double staining for β-galactosidase in combination with the neuronal marker NeuN (Millipore, Schwalbach, Germany), the astrocytic marker S-100B (Abcam, Cambridge UK), and the oligodendrocyte marker CNPase (Sigma Aldrich, Munich, Germany). ..

    Article Title: Transcranial Direct Current Stimulation Modulates Neurogenesis and Microglia Activation in the Mouse Brain
    Article Snippet: .. To exclude any animals with tDCS-induced cortical lesions, sections were stained for neuronal integrity using the neuronal marker NeuN (mouse monoclonal, 1 : 2000, cat# MAB377, Millipore, Germany). .. In order to assess the effect of tDCS on local inflammation, activated resident microglia expressing the ionized calcium binding adaptor molecule 1 (Iba1) were labeled with polyclonal rabbit anti-Iba1 (1 : 1000, cat# 019-19741, Wako, Germany).

    Article Title: Integration of hunger and hormonal state gates infant-directed aggression
    Article Snippet: RNAscope probes were Hcn1 (ACD, Cat. No. 423658), Hcn2 (Cat. No. 427009), Hcn3 (Cat. No. 551528) and Hcn4 (Cat. No. 421278). .. Immunostainings against the neuronal marker NeuN were subsequently performed (Millipore MAB377, 1:500). .. Images were acquired on a Vectra Polaris Automated Quantitative Pathology Imaging System (Akoya Biosciences) at 20· magnification.

    Article Title: Maternal immune activation differentially impacts mature and adult-born hippocampal neurons in male mice.
    Article Snippet: Schizophrenia is associated with deficits in the hippocampus, a brain area important for learning and memory.. The dentate gyrus (DG) of the hippocampus develops both before and after birth.. To study the relative contribution of mature and adult-born DG granule cells to disease etiology, we compared both cell populations in a mouse model of psychiatric illness resulting from maternal immune activation.

    Article Title: A protocol for preparation and transfection of rat entorhinal cortex organotypic cultures for electrophysiological whole-cell recordings
    Article Snippet: .. Immunofluorescent staining for the neuronal marker NeuN (1:200, Milipore, #ABN78) demonstrated that EC layers were well-preserved in slice cultures and that neurons were detected after being in culture for several days ( B). ..

    Article Title: Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) Deficiency Attenuates Phagocytic Activities of Microglia and Exacerbates Ischemic Damage in Experimental Stroke
    Article Snippet: .. Sections were colabeled with the neuronal marker NeuN (1:50; Millipore) or the CD11b microglial/monocyte marker (1:50; ab75476, Abcam), then reacted with fluorescently labeled secondary antibodies. .. Activated microglia and phagocytic microglia were also identified using peroxidase-labeled Griffonia simplicifolia isolectin-B4 (IB4) (10 g/ml; L5391, Sigma), or with CD68 rat antimouse monoclonal antibody (1:200; ab53444, Abcam), respectively, followed by diaminobenzidine (Vector Laboratories).

    Avidin-Biotin Assay:

    Article Title: Differential effects of immunotherapy with antibodies targeting α-synuclein oligomers and fibrils in a transgenic model of synucleinopathy
    Article Snippet: Immunohistochemistry, neuropathological and image analysis Analysis of α-syn accumulation was performed using vibratome-cut free-floating coronal brain sections (40 μm). .. Brain sections were incubated overnight at 4 °C with a polyclonal antibody against total α-syn (1:500, affinity-purified rabbit polyclonal, Millipore) ( Games et al., 2013 ; Masliah et al., 2000 ) in the presence or absence of proteinase K (PK; 10 μg/ml for 15 min) or non-immune IgG controls (background levels), a neuronal marker (NeuN, mouse monoclonal, Millipore 1:2500), an astroglial cell marker (glial fibrillary acidic protein [GFAP], mouse monoclonal, Millipore 1:2500), a microglial cell marker (Iba1; goat polyclonal, Abcam, 1:5000). followed by incubations with secondary antibodies biotinylated (1:100, Vector Laboratories, Inc., Burlin-game, CA), Avidin D-HRP (1:200, ABC Elite, Vector) and detection with 3,3′-diaminobenzidine ( Masliah et al., 2011 ). ..

    Isolation:

    Article Title: Microarray analysis of expression of cell death-associated genes in rat spinal cord cells exposed to cyclic tensile stresses in vitro
    Article Snippet: .. Previously we have documented [ ] that ~71% of cells isolated from the spinal cord using this methodology are positive for the neuronal marker NeuN (Chemicon International, Temecula, CA). ..

    Injection:

    Article Title: Prefrontal Cortical Dysfunction After Overexpression of Histone Deacetylase 1
    Article Snippet: .. ... To determine which of the major cell populations express the transgene (which was driven by a generic cytomegalovirus promoter) when packaged into the AAV9 capsid, we employed co-localization studies on brain sections of mice injected with the AAV9-LacZ reporter by double staining for β-galactosidase in combination with the neuronal marker NeuN (Millipore, Schwalbach, Germany), the astrocytic marker S-100B (Abcam, Cambridge UK), and the oligodendrocyte marker CNPase (Sigma Aldrich, Munich, Germany). ..

    Double Staining:

    Article Title: Prefrontal Cortical Dysfunction After Overexpression of Histone Deacetylase 1
    Article Snippet: .. ... To determine which of the major cell populations express the transgene (which was driven by a generic cytomegalovirus promoter) when packaged into the AAV9 capsid, we employed co-localization studies on brain sections of mice injected with the AAV9-LacZ reporter by double staining for β-galactosidase in combination with the neuronal marker NeuN (Millipore, Schwalbach, Germany), the astrocytic marker S-100B (Abcam, Cambridge UK), and the oligodendrocyte marker CNPase (Sigma Aldrich, Munich, Germany). ..

    Staining:

    Article Title: Transcranial Direct Current Stimulation Modulates Neurogenesis and Microglia Activation in the Mouse Brain
    Article Snippet: .. To exclude any animals with tDCS-induced cortical lesions, sections were stained for neuronal integrity using the neuronal marker NeuN (mouse monoclonal, 1 : 2000, cat# MAB377, Millipore, Germany). .. In order to assess the effect of tDCS on local inflammation, activated resident microglia expressing the ionized calcium binding adaptor molecule 1 (Iba1) were labeled with polyclonal rabbit anti-Iba1 (1 : 1000, cat# 019-19741, Wako, Germany).

    Article Title: A protocol for preparation and transfection of rat entorhinal cortex organotypic cultures for electrophysiological whole-cell recordings
    Article Snippet: .. Immunofluorescent staining for the neuronal marker NeuN (1:200, Milipore, #ABN78) demonstrated that EC layers were well-preserved in slice cultures and that neurons were detected after being in culture for several days ( B). ..

    Neutralization:

    Article Title: Maternal immune activation differentially impacts mature and adult-born hippocampal neurons in male mice.
    Article Snippet: Schizophrenia is associated with deficits in the hippocampus, a brain area important for learning and memory.. The dentate gyrus (DG) of the hippocampus develops both before and after birth.. To study the relative contribution of mature and adult-born DG granule cells to disease etiology, we compared both cell populations in a mouse model of psychiatric illness resulting from maternal immune activation.

    Blocking Assay:

    Article Title: Maternal immune activation differentially impacts mature and adult-born hippocampal neurons in male mice.
    Article Snippet: Schizophrenia is associated with deficits in the hippocampus, a brain area important for learning and memory.. The dentate gyrus (DG) of the hippocampus develops both before and after birth.. To study the relative contribution of mature and adult-born DG granule cells to disease etiology, we compared both cell populations in a mouse model of psychiatric illness resulting from maternal immune activation.

    Labeling:

    Article Title: Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) Deficiency Attenuates Phagocytic Activities of Microglia and Exacerbates Ischemic Damage in Experimental Stroke
    Article Snippet: .. Sections were colabeled with the neuronal marker NeuN (1:50; Millipore) or the CD11b microglial/monocyte marker (1:50; ab75476, Abcam), then reacted with fluorescently labeled secondary antibodies. .. Activated microglia and phagocytic microglia were also identified using peroxidase-labeled Griffonia simplicifolia isolectin-B4 (IB4) (10 g/ml; L5391, Sigma), or with CD68 rat antimouse monoclonal antibody (1:200; ab53444, Abcam), respectively, followed by diaminobenzidine (Vector Laboratories).



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